RNA

Part:BBa_K4950010:Design

Designed by: Lucas Ng   Group: iGEM23_PennState   (2023-10-12)


bFGF4 Riboswitch Insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.


Source

Designed by De Novo DNA Riboswitch Calculator

References

[1] Jellinek, D., Lynott, C. K., Rifkin, D. B., & Janjić, N. (1993). High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding. Proceedings of the National Academy of Sciences of the United States of America, 90(23), 11227–11231. https://doi.org/10.1073/pnas.90.23.11227