RNA
Part:BBa_K4950010:Design
Designed by: Lucas Ng Group: iGEM23_PennState (2023-10-12)
bFGF4 Riboswitch Insert
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.
Source
Designed by De Novo DNA Riboswitch Calculator
References
[1] Jellinek, D., Lynott, C. K., Rifkin, D. B., & Janjić, N. (1993). High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding. Proceedings of the National Academy of Sciences of the United States of America, 90(23), 11227–11231. https://doi.org/10.1073/pnas.90.23.11227